| Application | GC5 chemically competent cells are comparable to the popular DH5α™ strain and carry recA1 and endA1 mutations that aid in plasmid stability and improved quality of prepared plasmid DNA. The GC5 strain allows for beta-galactosidase α-complementation for blue/white screening and is T1 bacteriophage resistant. GC5 Competent Cells are offered as a high efficiency grade for subcloning and generation of cDNA libraries. |
| Other Notes | The transformation efficiency of GC5 Uni-packs (50 μl single use aliquots) is 1 × 109 cfu/μg pUC19 plasmid DNA. pUC19 Control DNA is provided. |
| | For more information on competent cell selection, the definition of genotypes and to view a presentation on competent cells, please visit www.sigma-aldrich.com/competentcells. |
| Legal Information | GC5 is a trademark of GeneChoice, Inc. |
